I in turn will do this to you: I will wreak misery upon you – consumption and fever, which cause the eyes to pine and the body to languish; you shall sow your seed to no purpose, for your enemies shall eat it. Leviticus 26:16 (The Israel BibleTM)
Huntington’s, Alzheimer’s, amyotrophic lateral sclerosis (ALS or Lou Gehrig’s disease) and many other devastating neurodegenerative diseases have something in common besides causing great suffering and death – they are all characterized by proteins (different ones for each disease) aggregating in neurons within the brain and nervous system.
Huntington’s, for example, is a rare, inherited disease that causes the progressive breakdown (degeneration) of nerve cells in the brain. Sadly, it has a broad impact on a person’s functional abilities and usually results in movement, thinking (cognitive) and psychiatric disorders. Now, scientists at the Technion-Israel Institute of Technology in Haifa have found that the cells have the mechanisms to clear those aggregates – they just fail to activate them. Their study has just been published in Nature Communications under the title “Differential roles for DNAJ isoforms in HTT-polyQ and FUS aggregation modulation revealed by chaperone screens.”
Proteins are the building blocks and the functioning units of our body. When the body needs something done, specific proteins are created to accomplish it. The code for the particular protein is read from the DNA, and the protein is built from sub-units called amino acids. It is then folded into the 3D shape it needs to assume. Other proteins, called “chaperones,” assist in this folding process. Aggregates form when certain proteins form incorrectly. Instead of performing the function they were supposed to perform, they attach to each other, creating sizeable clusters that not only are useless but also disrupt the cells’ normal functionality. Technion doctoral student Kinneret Rozales and M.D./Ph.D. student Amal Younis, working as part of the research group of Prof. Reut Shalgi, examined how the cells respond to the aggregates building up inside them. How can we know how a cell feels? We can’t ask it whether it is happy or in pain – but we can examine which genes the cell expresses. We know the cell would activate certain genes when it feels stressed. On the other hand, if everything is fine, those genes would not be activated. Some of what the cell does in response to stress is to activate specific chaperones, in an attempt to correct or remove misfolded proteins.
But which chaperones are activated and which ones are needed to solve the problem? A great many different chaperones are encoded in the human DNA. Rozales and Younis examined 66 of them in cells with Huntington or ALS-associated protein aggregates. Some chaperones, they found, only make things worse. But quite surprisingly, they also found chaperones that could eliminate the aggregates, curing the cell. The tools to cure the disease are already within us, encoded by our own DNA, the Haifa researchers discovered. Why then, if the necessary chaperones exist, don’t they cure patients’ cells before neurons degenerate? “It is not enough that the tools exist in the cell’s toolbox,” said Shalgi. “The cell needs to realize there is a problem, and then it needs to know which out of the many tools available to it, it should use to solve the problem.”
Unfortunately, the group found, this is where the bottleneck lies. In cells with Huntington-associated protein aggregates, the cells sensed there was a problem, and activated some stress-response chaperones, but not the correct ones. The cells did not know what was causing the stress, or what they should do to correct the situation. With ALS-associated aggregates, things were even worse; the cells did not realize that they need to activate chaperones at all and displayed no signs of stress. “The cell is a complicated system,” she added, explaining the surprising findings. “Think of your computer: when something is wrong, sometimes you do not realize it at first. It just responds a bit slower than it used to, perhaps, or it throws an error message that you ignore and forget. When you do realize something wrong – in the way of a blue screen or a refusal to start, you, or a technician on your behalf, attempt to diagnose and solve the problem. Sometimes the solution is found straight away, but other times it is something that you never encountered before, and you don’t know which driver needs to be installed, or piece of hardware needs to be replaced.”
It is the same, Shalgi said, with our cells. “They do not always realize there is a problem, or know how to solve it, even when they do in fact have the tools to do so. The good news is that since the ability is there, we hope future treatments can be developed to activate it and employ the body’s own tools to cure these debilitating neurodegenerative diseases.”